Refolding of thermally and urea-denatured ribonuclease A monitored by time-resolved FTIR spectroscopy.

@article{Reinstdler1996RefoldingOT,
  title={Refolding of thermally and urea-denatured ribonuclease A monitored by time-resolved FTIR spectroscopy.},
  author={Diane Reinst{\"a}dler and Heinz Fabian and Jan Backmann and Dieter Naumann},
  journal={Biochemistry},
  year={1996},
  volume={35 49},
  pages={15822-30}
}
We undertook a first detailed comparative analysis of the refolding kinetics of ribonuclease A (RNase A) by time-resolved Fourier transform infrared spectroscopy. The refolding process was initiated either by applying a temperature jump on the thermally denatured protein or by rapid dilution of a concentrated [13C]urea solution containing the chemically unfolded protein. The dead time of the injecting and mixing devices and the time-resolution of the spectrometer permitted us to monitor the… CONTINUE READING

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