Refolding of a bifunctional enzyme and its monofunctional fragment.


The renaturation of the bifunctional enzyme aspartokinase II-homoserine dehydrogenase II has been studied by using the reappearance of its two activities. The same kinetics of renaturation are obtained for the dehydrogenase (EC and the kinase activity (EC The mechanism of refolding of the enzyme apparently involves two steps, a folding… (More)


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