Refolding and purification of interferon-gamma in industry by hydrophobic interaction chromatography.

  title={Refolding and purification of interferon-gamma in industry by hydrophobic interaction chromatography.},
  author={Xindu Geng and Q. C. Bai and Yangjun Zhang and Xiang Li and Dan Wu},
  journal={Journal of biotechnology},
  volume={113 1-3},
A new technology for renaturation with simultaneous purification of the recombinant human interferon-gamma (rhIFN-gamma) in downstream of biotechnology is presented. The strategies to develop the new technology in industry scale were suggested. Based on chemical equilibrium and molecular interactions, the principle of rhIFN-gamma refolding by HPHIC was described. The kind of stationary and mobile phases were evaluated and found the former to contribute to the rhIFN-gamma refolding more than the… CONTINUE READING


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