[Refolding and purification of Plasmodium falciparum glutamate dehydrogenase fusion protein].

Abstract

OBJECTIVE To establish the method for renaturation and purification of the fusion protein of Plasmodium falfiparum (FCC1/HN ) glutamate dehydrogenase (GDH) with glutathione S-transferase (GST). METHODS The recombinant plasmid GDH/pGEX-4T-1, encoding the full-length GDH gene, was transformed into E.coli BL21 (DE3) to achieve IPTG-induced high expression of… (More)

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@article{Li2003RefoldingAP, title={[Refolding and purification of Plasmodium falciparum glutamate dehydrogenase fusion protein].}, author={Yan Li and Yun-shan Ning and Wenbo Hao and Li Li and Ming Li}, journal={Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA}, year={2003}, volume={23 12}, pages={1273-6} }