Reflection contrast microscopy

  title={Reflection contrast microscopy},
  author={I. Cornelese-ten Velde and J. Bonnet and Hans Tanke and Johan S. Ploem},
Reflection contrast microscopy (RCM) has proven to be a useful tool for the study of living cells (Ploem 1975). Due to the effective suppression of aspecific reflected light by polarization optics combined with a quarter lambda plate at the front lens of the objective, low intensity reflection signals originating from minor amounts of precipitated diaminobenzidine (DABox) in immunocytochemically stained specimens, can be made visible. RCM has been successfully applied in demonstrating single… CONTINUE READING