Refined structure of alpha beta-tubulin at 3.5 A resolution.

  title={Refined structure of alpha beta-tubulin at 3.5 A resolution.},
  author={Jan L{\"o}we and Hongbin Li and Kenneth H Downing and Eva Nogales},
  journal={Journal of molecular biology},
  volume={313 5},
We present a refined model of the alpha beta-tubulin dimer to 3.5 A resolution. An improved experimental density for the zinc-induced tubulin sheets was obtained by adding 114 electron diffraction patterns at 40-60 degrees tilt and increasing the completeness of structure factor amplitudes to 84.7 %. The refined structure was obtained using maximum-likelihood including phase information from experimental images, and simulated annealing Cartesian refinement to an R-factor of 23.2 and free R… CONTINUE READING
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