Refined protocol for generating monoclonal antibodies from single human and murine B cells.

@article{Ho2016RefinedPF,
  title={Refined protocol for generating monoclonal antibodies from single human and murine B cells.},
  author={Irvin Ho and Jeffrey J Bunker and Steven A Erickson and Karlynn E. Neu and Min Huang and Mario Cortese and Bali Pulendran and Patrick C. Wilson},
  journal={Journal of immunological methods},
  year={2016},
  volume={438},
  pages={
          67-70
        }
}
Generating monoclonal antibodies from single B cells is a valuable tool for characterizing the specificity and functional properties of humoral responses. We and others developed protocols that have facilitated major advances in our understanding of B cell development, tolerance, and effector responses to HIV and influenza. Here, we demonstrate various refinements and dramatically reduce the time required to produce recombinant antibodies. Further, we present new methods for cloning and… CONTINUE READING
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