Refilling of cortical calcium stores in Paramecium cells: in situ analysis in correlation with store-operated calcium influx.

@article{Mohamed2003RefillingOC,
  title={Refilling of cortical calcium stores in Paramecium cells: in situ analysis in correlation with store-operated calcium influx.},
  author={Isma{\"e}l Mohamed and Marc R Husser and Ivonne M. Sehring and Joachim Hentschel and Carsten Hentschel and Hasso Plattner},
  journal={Cell calcium},
  year={2003},
  volume={34 1},
  pages={87-96}
}
This is the first thorough study of refilling of a cortical calcium store in a secretory cell after stimulation in which we combined widely different methodologies. Stimulation of dense-core vesicle ("trichocysts") exocytosis in Paramecium involves a Ca(2+) -influx" superimposed to Ca(2+) -release from cortical stores ("alveolar sacs" (ASs)). In quenched-flow experiments, membrane fusion frequency rose with increasing [Ca(2+)](o) in the medium, from approximately 20-25% at [Ca(2+)](o) < or = 0… CONTINUE READING

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