Reference gene selection for normalization of PCR analysis in chicken embryo fibroblast infected with H5N1 AIV

@article{Yue2010ReferenceGS,
  title={Reference gene selection for normalization of PCR analysis in chicken embryo fibroblast infected with H5N1 AIV},
  author={Hua Yue and Xiao-wen Lei and Fa-long Yang and Ming-Yi Li and Cheng Tang},
  journal={Virologica Sinica},
  year={2010},
  volume={25},
  pages={425-431}
}
Chicken embryo fibroblasts (CEFs) are among the most commonly used cells for the study of interactions between chicken hosts and H5N1 avian influenza virus (AIV). In this study, the expression of eleven housekeeping genes typically used for the normalization of quantitative real-time PCR (QPCR) analysis in mammals were compared in CEFs infected with H5N1 AIV to determine the most reliable reference genes in this system. CEFs cultured from 10-day-old SPF chicken embryos were infected with 100… CONTINUE READING
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Avian influenza A virus ( H 5 N 1 ) outbreaks , Kuwait , 2007

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