Reference gene alternatives to Gapdh in rodent and human heart failure gene expression studies

@inproceedings{Brattelid2009ReferenceGA,
  title={Reference gene alternatives to Gapdh in rodent and human heart failure gene expression studies},
  author={Trond Brattelid and Lisbeth H. Winer and Finn Olav Levy and Knut Liest\ol and Ole Mathias Sejersted and Kristin Brevik Andersson},
  booktitle={BMC Molecular Biology},
  year={2009}
}
Quantitative real-time RT-PCR (RT-qPCR) is a highly sensitive method for mRNA quantification, but requires invariant expression of the chosen reference gene(s). In pathological myocardium, there is limited information on suitable reference genes other than the commonly used Gapdh mRNA and 18S ribosomal RNA. Our aim was to evaluate and identify suitable reference genes in human failing myocardium, in rat and mouse post-myocardial infarction (post-MI) heart failure and across developmental stages… CONTINUE READING
37 Citations
30 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 37 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 30 references

Similar Papers

Loading similar papers…