Reference Gene Selection for Quantitative PCR Studies in Sheep Neutrophils

@inproceedings{Vorachek2013ReferenceGS,
  title={Reference Gene Selection for Quantitative PCR Studies in Sheep Neutrophils},
  author={William R. Vorachek and Hugejiletu and Gerd Bobe and Jean A Hall},
  booktitle={International journal of molecular sciences},
  year={2013}
}
Reference genes are essential for studying mRNA expression with quantitative PCR (qPCR). We investigated 11 potential neutrophil reference genes (RPL19, GAPDH, ACTB, B2M, HPRT, G6PD, TFRC, PGK1, YWHAZ, SDHA and GYPC) for sheep under disease conditions of foot rot (FR) and with or without Se supplementation. Initial screening was based on gene expression level (<28 Cq cycles) and variability (SD < 1.5 Cq cycles) and excluded TFRC, GYPC and HPRT from further analysis. Expression stability of the… CONTINUE READING

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