Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera.

@article{Ritzenthaler2002ReevaluationOT,
  title={Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera.},
  author={Christophe Ritzenthaler and Andreas Nebenf{\"u}hr and Ali Movafeghi and Christiane Stussi-Garaud and Leila Behnia and Peter Pimpl and L Andrew Staehelin and David G Robinson},
  journal={The Plant cell},
  year={2002},
  volume={14 1},
  pages={237-61}
}
Brefeldin A (BFA) causes a block in the secretory system of eukaryotic cells by inhibiting vesicle formation at the Golgi apparatus. Although this toxin has been used in many studies, its effects on plant cells are still shrouded in controversy. We have reinvestigated the early responses of plant cells to BFA with novel tools, namely, tobacco Bright Yellow 2 (BY-2) suspension-cultured cells expressing an in vivo green fluorescent protein-Golgi marker, electron microscopy of high-pressure frozen… CONTINUE READING
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