Reductive cleavage of disulfide bridges in ribonuclease.

@article{Sela1957ReductiveCO,
  title={Reductive cleavage of disulfide bridges in ribonuclease.},
  author={M. Sela and F. H. White and C. Anfinsen},
  journal={Science},
  year={1957},
  volume={125 3250},
  pages={
          691-2
        }
}
This article was the first of many studies that were generally concerned with the eventual total synthesis of the protein. It contained several original observations that would lead to the conclusion that the refolding of the ribonuclease molecule after full denaturation by reductive cleavage of its four disulfide bonds required that only one of the one hundred five possible pairings of eight sulfhydryl groups to form four disulfide linkages take place. 
View on PubMed
profiles.nlm.nih.gov
231 Citations
Highly Influential Citations
2
Background Citations
28
Methods Citations
1

Topics from this paper

      231 Citations

      Citation Type

      Citation Type

      SOME RELATIONSHIPS OF STRUCTURE TO FUNCTION IN RIBONUCLEASE
      • 45
      • PDF
      A comparison of the physical chemical properties of oxidized and reduced alkylated ribonuclease.
      • 61
      IMMUNOCHEMICAL REACTIONS INVOLVING RIBONUCLEASE *
      • 35
      Protection of Thiols
      • 8
      The role of disulfide bonds in the protein structure. Conformational studies on reduced ribonuclease and lysozyme.
      • 24
      The reductive cleavage of disulfide bonds and its application to problems of protein structure.
      • 111
      Cleavage of one specific disulfide bond in papain.
      • 61
      • PDF
      The correlation of ribonuclease activity with specific aspects of tertiary structure.
      • 170
      THE RELATION OF STRUCTURE TO ENZYMATIC ACTIVITY IN RIBONUCLEASE *
      • 57
      The reformation of disulfide bridges in proteins.
      • 38
      ...
      1
      2
      3
      4
      5
      ...

      References

      SHOWING 1-10 OF 11 REFERENCES
      Studies on the gross structure, cross-linkages, and terminal sequences in ribonuclease.
      • 313
      • PDF
      Peptides obtained by tryptic hydrolysis of performic acid-oxidized ribonuclease.
      • 435
      • PDF
      The activity of ribonuclease after digestion with carboxypeptidase.
      • 18
      The separation of N-2:4-dinitrophenly amino-acids on paper chromatograms.
      • 212
      • PDF
      Poly-L-cysteine
      • 69
      The Reduction of the Disulfide Bonds of Insulin
      • 34
      On leave of absence from the Weizmann Institute of Science, Rebovoth, Israel
      • Ckem. Sot
      • 1954
      Spectrophotometric Study of the Reaction of Protein Sulfhydryl Groups with Organic Mercurials
      • 1,225
      Techniques and Reagents for Paper Chromatography
      • 550
      [Paper partition chromatography of dinitrophenyl amino acids].
      • 2
      ...
      1
      2
      ...