Reduction of the developmental competence of sheep oocytes by inhibition of LH pulses during the follicular phase with a GnRH antagonist.

@article{Oussaid1999ReductionOT,
  title={Reduction of the developmental competence of sheep oocytes by inhibition of LH pulses during the follicular phase with a GnRH antagonist.},
  author={B Oussaid and Jean C. Mariana and Nati Poulin and J. -L. Fontaine and Patrick Lonergan and Jean François Beckers and Yves Cogni{\'e}},
  journal={Journal of reproduction and fertility},
  year={1999},
  volume={117 1},
  pages={71-7}
}
A GnRH antagonist (Antarelix) treatment was used during the breeding season of Romanov ewes, to investigate whether LH pulses are required the day before the preovulatory surge for normal early embryo development in vivo (Expt 1) and in vitro (Expt 2). In Expt 1, at the onset of oestrus after removal of a fluorogestone acetate sponge, group A0.5 (n = 22) received a subcutaneous injection of 0.5 mg Antarelix, and ovulation was induced with an intravenous injection of 3 mg pig LH 24 h later. The… CONTINUE READING