Reduction of marker discrimination in transductional recombination

  title={Reduction of marker discrimination in transductional recombination},
  author={Millicent Masters and Barbara J. Newman and Christine M. Henry},
  journal={Molecular and General Genetics MGG},
The recovery of phage P1 mediated transductants varies with the marker selected in a manner which cannot be fully accounted for by dosage differences in the donor gene population. This variation in transduction frequency is due primarily to recombinational discrimination in the recipient cell. We show here that increasing the intracellular level of recA protein, which might be expected to increase the contribution of recF mediated events to recombinant formation, decreases this discrimination… CONTINUE READING

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