Reduction of immunoreactivity of bovine beta-lactoglobulin upon combined physical and proteolytic treatment.

@article{Bonomi2003ReductionOI,
  title={Reduction of immunoreactivity of bovine beta-lactoglobulin upon combined physical and proteolytic treatment.},
  author={Francesco Bonomi and Alessandro Fiocchi and Hanne Fr\okiaer and Antonella Gaiaschi and Stefania Iametti and Claudio Poiesi and Patrizia Rasmussen and Patrizia Restani and Pierpaolo Rovere},
  journal={The Journal of dairy research},
  year={2003},
  volume={70 1},
  pages={51-9}
}
Bovine beta-lactoglobulin was hydrolyzed with trypsin or chymotrypsin before, during and after treatment at 600 MPa and pH 6.8 for 10 min at 30, 37 and 44 degrees C. The extent of beta-lactoglobulin hydrolysis under pressure was noticeably higher than at atmospheric pressure, particularly when chymotrypsin was used. Addition of proteases at ambient pressure to previously pressure-treated beta-lactoglobulin gave only a modest increase in proteolysis with respect to the untreated protein… CONTINUE READING