Reduction of dye coupling in glial cultures by microinjection of antibodies against the liver gap junction polypeptide.

@article{Dudek1988ReductionOD,
  title={Reduction of dye coupling in glial cultures by microinjection of antibodies against the liver gap junction polypeptide.},
  author={F. Edward Dudek and Valentin K. Gribkoff and James E. Olson and Effiot L. Hertzberg},
  journal={Brain research},
  year={1988},
  volume={439 1-2},
  pages={275-80}
}
Intracellular injection of antibodies to the 27-kDa liver gap junction polypeptide have been shown previously to uncouple pairs of cultured mammalian hepatocytes, cardiac myocytes, and ganglionic neurons (Proc. Natl. Acad. Sci. U.S.A., 82 (1985) 2412-2416). In confluent primary cultures of astrocytes, similar injections significantly reduced dye coupling for cells closer than 80 micron to the injected glial cell. Western blots identified a 27-kDa protein in extracts of the astrocyte cultures… CONTINUE READING