Reduction of caspase-8 and -9 cleavage is associated with increased c-FLIP and increased binding of Apaf-1 and Hsp70 after neonatal hypoxic/ischemic injury in mice overexpressing Hsp70.

@article{Matsumori2006ReductionOC,
  title={Reduction of caspase-8 and -9 cleavage is associated with increased c-FLIP and increased binding of Apaf-1 and Hsp70 after neonatal hypoxic/ischemic injury in mice overexpressing Hsp70.},
  author={Yasuhiko Matsumori and Frances J. Northington and Shwuhuey M. Hong and Takamasa Kayama and R. Ann Sheldon and Zinaida S Vexler and Donna M. Ferriero and Philip R. Weinstein and Jialing Liu},
  journal={Stroke},
  year={2006},
  volume={37 2},
  pages={
          507-12
        }
}
BACKGROUND AND PURPOSE Caspase-8 and caspase-9 are essential proteases of the extrinsic and intrinsic apoptotic pathways, respectively. We investigated whether neuroprotection associated with overexpression of heat-shock protein 70 (Hsp70), a natural cellular antiapoptotic protein, is mediated by caspase-8 and caspase-9 signaling in the neonatal mouse brain after hypoxia/ischemia (H/I) injury. METHODS Postnatal day 7 transgenic mice overexpressing rat Hsp70 (Hsp70 Tg) and their wild-type (Wt… Expand
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References

SHOWING 1-10 OF 27 REFERENCES
Hsp70 Overexpression Sequesters AIF and Reduces Neonatal Hypoxic/Ischemic Brain Injury
  • Y. Matsumori, Shwuhuey M. Hong, +7 authors Jialing Liu
  • Biology, Medicine
  • Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
  • 2005
TLDR
The results suggest that interaction between Hsp70 and AIF might have reduced downstream events leading to cell death, including the reduction of nuclear AIF translocation in the neonatal brains of Hsp 70 Tg mice after H/I. Expand
Heat-shock protein 70 antagonizes apoptosis-inducing factor
TLDR
The data indicate that Hsp70 can inhibit apoptosis by interfering with target proteins other than Apaf-1, one of which is AIF, which is a mitochondrial intermembrane flavoprotein. Expand
Heat-shock protein 70 inhibits apoptosis by preventing recruitment of procaspase-9 to the Apaf-1 apoptosome
TLDR
It is shown, using a cell-free system, that Hsp70 prevents cytochrome c/dATP-mediated caspase activation, but allows the formation of Apaf-1 oligomers, which suppresses apoptosis by directly associating with Apf-1 and blocking the assembly of a functional apoptosome. Expand
Negative regulation of the Apaf-1 apoptosome by Hsp70
TLDR
It is proposed that resistance to apoptosis exhibited by stressed cells and some tumours, which constitutively express high levels of Hsp70, may be due in part to modulation of Apaf-1 function by HSp70. Expand
Reduced Apoptosis and Cytochrome c–Mediated Caspase Activation in Mice Lacking Caspase 9
TLDR
Results indicate that Casp9 is a critical upstream activator of the caspase cascade in vivo, as indicated by the absence of Casp3-like cleavage and the restoration of cytochrome c-mediated cleavage after addition of in vitro-translated Casp 9. Expand
Ordering the Cytochrome c–initiated Caspase Cascade: Hierarchical Activation of Caspases-2, -3, -6, -7, -8, and -10 in a Caspase-9–dependent Manner
TLDR
Six additional caspases (caspases-2, -3, -6, -7, -8, and -10) are processed in cell-free extracts in response to cytochrome c, and that three others failed to be activated under the same conditions. Expand
Mice overexpressing rat heat shock protein 70 are protected against cerebral infarction
TLDR
It is suggested that HSP70 can markedly protect the brain against ischemic damage and that approaches aimed at inducing HSP 70 may lead to new therapeutic interventions in cerebrovascular injuries. Expand
Neonatal mice lacking functional Fas death receptors are resistant to hypoxic–ischemic brain injury
TLDR
It is concluded that Fas signaling via both extrinsic and intrinsic caspase cascades causes brain injury following neonatal HI in a region-dependent manner. Expand
Differential Requirement for Caspase 9 in Apoptotic Pathways In Vivo
TLDR
Comparison of the requirement for Casp9 and Casp3 in different apoptotic settings indicates the existence of at least four different apoptosis pathways in mammalian cells. Expand
Many Mechanisms for Hsp70 Protection From Cerebral Ischemia
TLDR
Overexpression of inducible Hsp70 has been shown to provide protection from cerebral ischemia both in animal models of stroke and in cell culture models, and several new mechanisms by which HSp70 can prevent cell death have been described. Expand
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