Reduction of O6-alkylguanine-DNA alkyltransferase activity in HeLa cells treated with O6-alkylguanines.

@article{Dolan1985ReductionOO,
  title={Reduction of O6-alkylguanine-DNA alkyltransferase activity in HeLa cells treated with O6-alkylguanines.},
  author={M. Eileen Dolan and Kazushige Morimoto and Anthony E. Pegg},
  journal={Cancer research},
  year={1985},
  volume={45 12 Pt 1},
  pages={6413-7}
}
Exposure of HeLa cells to 0.2 mM O6-methylguanine for 4 h or longer led to a 70-80% loss in the activity of the DNA-repair protein, O6-alkylguanine-DNA alkyltransferase. The decline in alkyltransferase activity brought about by O6-methylguanine was reversible on removing the base but at least 48 h were required for complete restoration. This loss of activity could also be brought about by other O6-alkylguanines including ethyl, n-propyl, and n-butyl, but the isopropyl and 2-hydroxyethyl… CONTINUE READING
21 Citations
32 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 21 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 32 references

Repair of 0'3K

  • J. R. Mehta, D. B. Ludlum, A. Renard, W. G. Veriy
  • Morimoto and A. E. Pegg, unpublishedobservations…
  • 1985

0eMethylguanine-DNAmethyltransferasein human cells

  • D. B. Yarosh, M. Rice, R. S. Day, Ill, R. S. Foote, S. Mitra
  • Mutât. Res.,
  • 1984

Alkylationof the O 6 of guanine is only one of many chemicalevents that may initiate carcinogenesis

  • P. Karran, S. A. andWilliams
  • Cancer Invest .
  • 1984

Alkylationof the O6of guanine is only one of many chemicalevents that may initiate carcinogenesis.Cancer Invest., 2:233-238,1984

  • B. Singer
  • 1984

Comparison of rates of repair of O 6 - alkylguaninesin DNA by rat liver and bacterial O * - alkykjuanine - DNA - alkyltransferase

  • J. Domoradzki, A. E. Pegg, M. E. Dolan, J. J. McCormtek
  • Cancer Res .
  • 1984

Correlation between Oe - methylguanine - DNA - methyltransferaseactivity and resistance of human cells to the cytotoxic and mutagenic effect of W - methyl - N - nitro - W - nitrosoguanidine

  • A. E. Pegg
  • Carcinogenesis ( Lond . )
  • 1984

Similar Papers

Loading similar papers…