Reduction of Dehydroascorbic Acid by Animal Tissues

@article{Hughes1964ReductionOD,
  title={Reduction of Dehydroascorbic Acid by Animal Tissues},
  author={Robert E. Hughes},
  journal={Nature},
  year={1964},
  volume={203},
  pages={1068-1069}
}
  • R. Hughes
  • Published 5 September 1964
  • Chemistry, Medicine
  • Nature
IT has long been known that an enzyme catalysing the reduction of dehydro-L-ascorbic acid (DHA) by glutathione to ascorbic acid occurs in plant tissues1–3. It can also be shown that DHA is in part reduced to ascorbic acid by animal tissues, although until recently no clear evidence was available to suggest that this is an enzymatic reaction. Recently, however, it has become clear that the reduction of DHA by hæmolysates of human erythrocytes occurs at a rate greater than can be accounted for in… Expand
The Passage of Vitamin C across the Erythrocyte Membrane
Summary In all the species examined dehydroascorbic acid entered the erythrocyte considerably more rapidly than ascorbic acid (vitamin C). The uptake was pH‐dependent but did not appear to beExpand
Regeneration of Ascorbic Acid by Rat Colon
  • J. Choi, R. C. Rose
  • Chemistry, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1989
TLDR
The present results indicate that the mucosa reduced dehydro-l-ascorbic acid by a cytosolic enzyme that uses NADPH as a hydrogen donor. Expand
Glutathione-dependent ascorbate recycling activity of rat serum albumin.
TLDR
Results support the suggestion that serum albumin acts as an antioxidant and exerts a significant glutathione-dependent DHAA-reductase activity that may be important in the physiologic recycling of ascorbic acid. Expand
Glutathione: dehydroascorbate oxidoreductases.
TLDR
This chapter focuses on the dehydroascorbate oxidoreductases of glutathione, which have been purified from numerous sources, including rat liver, pig liver, calf thymus, rabbit bone marrow, human placenta, and human erythrocytes. Expand
An ascorbate-mediated transmembrane-reducing system of the human erythrocyte.
TLDR
Work described in this paper suggests that the transmembrane reduction is effected by ascorbic acid, a compound in its oxidized form (dehydroascorbate) rapidly enters the cell and accomplishes the non-enzymatic reduction of ferricyanide. Expand
Cerebral metabolism of oxidized ascorbate
TLDR
The results suggest that a cytosolic component of cerebrum regenerates ascorbic acid in a step that preferentially uses GSH and NADPH as reducing cofactors, and at least one form of DHAA reductase exists in brain. Expand
UPTAKE AND REDUCTION OF OXIDIZED AND REDUCED ASCORBATE BY HUMAN LEUKOCYTES
TLDR
It is proposed that human leukocytes take up ascorbate as DHA (AA after oxidation to DHA) and reduce it promptly to AA, and that HMS stimulation upon incubation with either AA or DHA is a result of DHA reduction. Expand
A reappraisal of leukocyte dehydroascorbate reductase.
TLDR
It is found that in human leukocytes, dehydroascorbate reduction does not occur enzymatically, and neither neutrophil nor chronic lymphocytic leukemia lymphocyte extracts had detectable activity. Expand
Human cell dehydroascorbate reductase. Kinetic and functional properties.
TLDR
Rates of dehydroascorbate uptake by intact human neutrophils, monocytes, lymphocytes and cultured fibroblasts were proportional to cytosol dehydroasorbate reductase activities, suggesting that dehydroASorbate reduction during dehydroASCorbate absorption by these cells may be entirely enzymatic. Expand
A ‘dehydroascorbic acid reductase’ factor in guinea-pig tissues
Dans des tissus de cobayes il existe une protéine capable de catalyser la réduction de l'acide déhydrascorbique en acide ascorbique. Sa distribution, sa purification, sa spécificité et son action enExpand
...
1
2
3
4
5
...

References

SHOWING 1-6 OF 6 REFERENCES
The reduction of dehydroascorbic acid by human erythrocytes.
TLDR
The findings suggest that an enzyme other than glutathione reductase is involved in the reduction of DHA by human erythrocytes, similar to that occurring in simple solution with similar DHA and GSH concentrations. Expand
THE REDUCTION OF DEHYDROASCORBIC ACID BY HAEMOLYSATES OF PERNICIOUS ANAEMIA ERYTHROCYTES.
  • R. Hughes, G. Kilpatrick
  • Chemistry, Medicine
  • Clinica chimica acta; international journal of clinical chemistry
  • 1964
TLDR
A reduction in the activity of this DHA reductase system could contribute towards the lowered plasma levels of ascorbic acid known to occur in pernicious anaemia. Expand
Purification and properties of dehydroascorbic acid reductase of peas (Pisum sativum).
TLDR
Addition of dihydrocozymase to the reductase system did not increase the reduction of dehydro- l -ascorbic acid, and cysteine and thioglycolic acid used as substrates did not replace glutathione as hydrogen donors. Expand
The use of homocysteine in the estimation of dehydroascorbic acid.
  • R. Hughes
  • Chemistry, Medicine
  • The Biochemical journal
  • 1956
TLDR
An account is given of a new method, based on the two findings that homocysteine rapidly reduces dehydroascorbic acid at pH 7 0, and that under certain simple conditions 2:6-dichlorophenolindophenol can be used to estimate ascorbic acid in the presence of homocy steine without interference from the latter compound. Expand
Studies on ribonuclease in tobacco leaves. I. Purification and properties.
TLDR
A ribonuclease was purified from tobacco leaves and the properties studied had a pH optimum of 5.1, was fairly heat stable and digested RNA to an extent of 98.8%. Expand
The system dehydroascorbic acid-glutathione.
  • E. M. Crook
  • Biology, Medicine
  • The Biochemical journal
  • 1941