Reduction of Dehydroascorbic Acid by Animal Tissues

@article{Hughes1964ReductionOD,
  title={Reduction of Dehydroascorbic Acid by Animal Tissues},
  author={Robert E. Hughes},
  journal={Nature},
  year={1964},
  volume={203},
  pages={1068-1069}
}
  • R. Hughes
  • Published 5 September 1964
  • Biology
  • Nature
IT has long been known that an enzyme catalysing the reduction of dehydro-L-ascorbic acid (DHA) by glutathione to ascorbic acid occurs in plant tissues1–3. It can also be shown that DHA is in part reduced to ascorbic acid by animal tissues, although until recently no clear evidence was available to suggest that this is an enzymatic reaction. Recently, however, it has become clear that the reduction of DHA by hæmolysates of human erythrocytes occurs at a rate greater than can be accounted for in… 

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References

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THE ability of tissue extracts from plants and animals, either to inhibit the oxidation of ascorbic acid (hereafter 'AA'), or to reduce added dehydroascorbic acid ('DHA'), is well known. In his

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TLDR
An account is given of a new method, based on the two findings that homocysteine rapidly reduces dehydroascorbic acid at pH 7 0, and that under certain simple conditions 2:6-dichlorophenolindophenol can be used to estimate ascorbic acid in the presence of homocy steine without interference from the latter compound.

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