Reduction in C-terminal amidated species of recombinant monoclonal antibodies by genetic modification of CHO cells

@inproceedings{kulj2014ReductionIC,
  title={Reduction in C-terminal amidated species of recombinant monoclonal antibodies by genetic modification of CHO cells},
  author={Mihaela {\vS}kulj and Dejan Pezdirec and Dominik Gaser and Marko Kreft and Robert Zorec},
  booktitle={BMC biotechnology},
  year={2014}
}
BACKGROUND During development of recombinant monoclonal antibodies in Chinese hamster ovary (CHO) cells, C-terminal amidated species are observed. C-terminal amidation is catalysed by peptidylglycine α-amidating monooxygenase (PAM), an enzyme known to be expressed in CHO cells. The significant variations between clones during clone selection, and the relatively high content of amidated species (up to 15%) in comparison to reference material (4%), led us to develop a cell line with reduced… CONTINUE READING
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