Reduction and alkylation of proteins in preparation of two-dimensional map analysis: why, when, and how?

@article{Herbert2001ReductionAA,
  title={Reduction and alkylation of proteins in preparation of two-dimensional map analysis: why, when, and how?},
  author={Ben R. Herbert and Marina Galvani and Mahmoud Hamdan and Erna Olivieri and J P MacCarthy and Sven Pedersen and Pier Giorgio Righetti},
  journal={Electrophoresis},
  year={2001},
  volume={22 10},
  pages={2046-57}
}
The standard procedure adopted up to the present in proteome analysis calls for just reduction prior to the isoelectric focusing/immobilized pH gradient (IEF/IPG) step, followed by a second reduction/alkylation step in between the first and second dimension, in preparation for the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) step. This protocol is far from being optimal. It is here demonstrated, by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF… CONTINUE READING