Reducing time and increasing sensitivity in sample preparation for adherent mammalian cell metabolomics.

@article{Lorenz2011ReducingTA,
  title={Reducing time and increasing sensitivity in sample preparation for adherent mammalian cell metabolomics.},
  author={Matthew A. Lorenz and Charles F. Burant and Robert T Kennedy},
  journal={Analytical chemistry},
  year={2011},
  volume={83 9},
  pages={3406-14}
}
A simple, fast, and reproducible sample preparation procedure was developed for relative quantification of metabolites in adherent mammalian cells using the clonal β-cell line INS-1 as a model sample. The method was developed by evaluating the effect of different sample preparation procedures on high performance liquid chromatography- mass spectrometry quantification of 27 metabolites involved in glycolysis and the tricarboxylic acid cycle on a directed basis as well as for all detectable… CONTINUE READING
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