Reducing cellular autofluorescence in flow cytometry: an in situ method.

  title={Reducing cellular autofluorescence in flow cytometry: an in situ method.},
  author={Victoria L Mosiman and Bruce K. Patterson and L Canterero and Charles L. Goolsby},
  volume={30 3},
Cellular autofluorescence affects the sensitivity of flow cytometric assays by interfering with detection of low level specific fluorescence. These detection limits increase with use of protocols, such as thermocycling and fluorescent in-situ hybridization (FISH), that can increase intrinsic cellular fluorescence to 5,000-20,000 fluorescein isothiocyanate (FITC) equivalents. In order to improve signal to noise ratios when using FITC labeled probes in these procedures, we employed a method using… CONTINUE READING


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