Reduced synthesis of [14C]mannosyl oligosaccharide-lipid by membranes prepared from concanavalin A-resistant Chinese hamster ovary cells.

Abstract

We compared the synthesis of mannosyl oligosaccharide-lipid by membranes of wild type and concanavalin A-resistant Chinese hamster ovary cells during incubations with GDP-[14C]mannose and UDP-N-acetylglucosamine. The membranes prepared from exponentially growing mutant cells incorporated 10- to 70-fold less [14C]mannose into oligosaccharide-lipid than did… (More)

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