Reduced substratum adhesion and decreased expressions of 1 and 4 integrins in human breast cancer cells with a property of anchorage-independent growth.

@article{Suzuki1999ReducedSA,
  title={Reduced substratum adhesion and decreased expressions of 1 and 4 integrins in human breast cancer cells with a property of anchorage-independent growth.},
  author={Kenji Suzuki and Kazuhide Takahashi},
  journal={International journal of oncology},
  year={1999},
  volume={14 5},
  pages={897-904}
}
The proliferation of normal human breast epithelial (HBE) cells was found to be dependent on their attachment on a solid culture substratum, whereas human breast cancer MCF-7 cells could grow in soft agar. The attachment of the dissociated single HBE cells to the substratum was complete within 4 h after replating, and most of the cells once attached were detached from the substratum by incubation with trypsin for 30 min. In contrast, the attachment of MCF-7 cells to the substratum proceeded… CONTINUE READING