Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose.

@article{Jeppsson2002ReducedOP,
  title={Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose.},
  author={Marie Jeppsson and Bj{\"o}rn Johansson and B{\"a}rbel Hahn-H{\"a}gerdal and Marie F. Gorwa-Grauslund},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 4},
  pages={1604-9}
}
In recombinant, xylose-fermenting Saccharomyces cerevisiae, about 30% of the consumed xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since xylose reductase uses both NADPH and NADH, while xylitol dehydrogenase uses only NAD(+). In this study we increased the ethanol yield and decreased the xylitol yield by lowering the flux through the NADPH-producing pentose phosphate pathway. The pentose phosphate pathway was blocked either by disruption of the GND1 gene… CONTINUE READING
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