Reduced glutathione and Trolox (vitamin E) as extender supplements in cryopreservation of red deer epididymal spermatozoa.

@article{AnelLpez2012ReducedGA,
  title={Reduced glutathione and Trolox (vitamin E) as extender supplements in cryopreservation of red deer epididymal spermatozoa.},
  author={L Anel-L{\'o}pez and Manuel {\'A}lvarez-Rodr{\'i}guez and Olga Garc{\'i}a-{\'A}lvarez and Mercedes Prudencio {\'A}lvarez and Alejandro Maroto-Morales and Luis Anel and P L P{\'e}rez de Paz and Jos{\'e} Juli{\'a}n Garde and F Mart{\'i}nez-Pastor},
  journal={Animal reproduction science},
  year={2012},
  volume={135 1-4},
  pages={37-46}
}
The use of assisted reproductive techniques in cervids is increasing as the commercial use of these species increase. We have tested the suitability of the antioxidants Trolox and reduced glutathione (GSH) for freezing red deer epididymal spermatozoa, aiming at improving post-thawing quality. Samples from 19 stags were frozen in a TES-Tris-fructose extender (20% egg yolk, 8% glycerol), with 1 or 5 mM of antioxidant. Motility (CASA), lipoperoxidation (malondialdehyde -MDA- production), membrane… CONTINUE READING
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