Reduced expression of the insulin receptor in mouse insulinoma (MIN6) cells reveals multiple roles of insulin signaling in gene expression, proliferation, insulin content, and secretion.

@article{Ohsugi2005ReducedEO,
  title={Reduced expression of the insulin receptor in mouse insulinoma (MIN6) cells reveals multiple roles of insulin signaling in gene expression, proliferation, insulin content, and secretion.},
  author={Mitsuru Ohsugi and Corentin Cras-M{\'e}neur and Yiyong Zhou and Ernesto Bernal-Mizrachi and James D Johnson and Dan S. Luciani and Kenneth S. Polonsky and M. Alan Permutt},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 6},
  pages={4992-5003}
}
The role of insulin signaling in pancreatic beta cells has become increasingly apparent. Stably transformed insulinoma cell lines (MIN6) were created with small interfering RNA resulting in the reduction of insulin receptor (IR) expression up to 80% (insulin receptor knockdown, IRKDDelta80). Functionally perturbed IR signaling was confirmed with the absence of insulin-stimulated insulin receptor substrate 1 tyrosine phosphorylation. Additionally, Akt phosphorylation was reduced and responded… CONTINUE READING