Reduced activity of alkaline phosphatase due to host-guest interactions with humic superstructures.

Abstract

Nuclear Magnetic Resonance (NMR) spectroscopy was applied to directly study the interactions between the alkaline phosphatase enzyme (AP) and two different humic acids from a volcanic soil (HA-V) and a Lignite deposit (HA-L). Addition of humic matter to enzyme solutions caused signals broadening in (1)H-NMR spectra, and progressive decrease and increase of… (More)
DOI: 10.1016/j.chemosphere.2013.07.015

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