Reduced Oxidative Pentose Phosphate Pathway Flux in Recombinant Xylose-Utilizing Saccharomyces cerevisiae Strains Improves the Ethanol Yield from Xylose

@article{Jeppsson2002ReducedOP,
  title={Reduced Oxidative Pentose Phosphate Pathway Flux in Recombinant Xylose-Utilizing Saccharomyces cerevisiae Strains Improves the Ethanol Yield from Xylose},
  author={M. Jeppsson and B. Johansson and B. Hahn-Haegerdal and M. Gorwa-Grauslund},
  journal={Applied and Environmental Microbiology},
  year={2002},
  volume={68},
  pages={1604 - 1609}
}
  • M. Jeppsson, B. Johansson, +1 author M. Gorwa-Grauslund
  • Published 2002
  • Medicine, Biology
  • Applied and Environmental Microbiology
  • ABSTRACT In recombinant, xylose-fermenting Saccharomyces cerevisiae, about 30% of the consumed xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since xylose reductase uses both NADPH and NADH, while xylitol dehydrogenase uses only NAD+. In this study we increased the ethanol yield and decreased the xylitol yield by lowering the flux through the NADPH-producing pentose phosphate pathway. The pentose phosphate pathway was blocked either by disruption of the… CONTINUE READING

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