Redox titration of multiple protein phosphorylations in pea chloroplast thylakoids

@inproceedings{Silverstein1993RedoxTO,
  title={Redox titration of multiple protein phosphorylations in pea chloroplast thylakoids},
  author={Todd P. Silverstein and L T Cheng and John F. Allen},
  year={1993}
}
Redox titrations were carried out on the protein kinase reactions of isolated pea chloroplast thylakoid membranes. Of the 13 phosphoproteins observed by autoradiography, all were found to titrate with the same midpoint potential of around +40 mV. Eleven proteins, including LHCII, D1, D2, CP43, a 9 kDa and a 55 kDa protein, were phosphorylated under reducing conditions, with a midpoint potential of Em = +38 ± 4 mV, n = 0.95 ± 0.06. Two other proteins, including one at 63 kDa, were phosphorylated… CONTINUE READING

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