Redox-sensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the cellular glutathione redox buffer.

@article{Meyer2007RedoxsensitiveGI,
  title={Redox-sensitive GFP in Arabidopsis thaliana is a quantitative biosensor for the redox potential of the cellular glutathione redox buffer.},
  author={Andreas J. Meyer and Thorsten Brach and Laurent Marty and Susanne Kreye and Nicolas Rouhier and Jean-Pierre Jacquot and R{\"u}diger Hell},
  journal={The Plant journal : for cell and molecular biology},
  year={2007},
  volume={52 5},
  pages={
          973-86
        }
}
  • A. Meyer, T. Brach, +4 authors R. Hell
  • Published 2007
  • Biology, Medicine
  • The Plant journal : for cell and molecular biology
The cellular glutathione redox buffer is assumed to be part of signal transduction pathways transmitting environmental signals during biotic and abiotic stress, and thus is essential for regulation of metabolism and development. Ratiometric redox-sensitive GFP (roGFP) expressed in Arabidopsis thaliana reversibly responds to redox changes induced by incubation with H(2)O(2) or DTT. Kinetic analysis of these redox changes, combined with detailed characterization of roGFP2 in vitro, shows that… Expand
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