Redox regulation of human Rac1 stability by the proteasome in human aortic endothelial cells.

@article{Kovacic2001RedoxRO,
  title={Redox regulation of human Rac1 stability by the proteasome in human aortic endothelial cells.},
  author={Herv{\'e} Kovacic and Kaikobad Irani and Pascal J. Goldschmidt-Clermont},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 49},
  pages={45856-61}
}
Rac1 has been shown to activate a NADPH oxidase complex producing superoxide anions in a variety of mammalian cell types. We evaluated the impact of Rac1-induced reactive oxygen species production on the turnover of Rac1 itself in human aortic endothelial cells. The concentration of a constitutively active mutant of Rac1 (Rac1(V12)) was increased by treatment of the cells with diphenylene iodinium (DPI), an inhibitor of the NADPH oxidase. Such an effect was not observed for the dominant… CONTINUE READING