Redox capacity of cells affects inactivation of glutathione reductase by nitrosative stress.

@article{Fujii2000RedoxCO,
  title={Redox capacity of cells affects inactivation of glutathione reductase by nitrosative stress.},
  author={Takuma Fujii and Rieko Hamaoka and Junichi Fujii and Naoyuki Taniguchi},
  journal={Archives of biochemistry and biophysics},
  year={2000},
  volume={378 1},
  pages={123-30}
}
Glutathione reductase (GR) plays a pivotal role in maintaining glutathione (GSH) in its reduced form. We have isolated a cDNA for rat GR and constructed a baculovirus system to produce recombinant GR on a large scale. This protein was purified by simple, two-step chromatographic procedure using DE52 and 2',5'-ADP Sepharose. Tissue distributions of GR were examined by Northern and Western blotting with a rabbit antibody to purified GR. GR was expressed in the order of reactivity; kidney, colon… CONTINUE READING

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