Redox and hormone profiling of a Nicotiana tabacum dedifferentiated protoplast culture suggests a role for a cytokinin and gibberellin in plant totipotency

@article{Jayaraman2015RedoxAH,
  title={Redox and hormone profiling of a Nicotiana tabacum dedifferentiated protoplast culture suggests a role for a cytokinin and gibberellin in plant totipotency},
  author={Jayaraj Jayaraman and Javier A Miret and Sergi Munn{\'e}-Bosch and Paul P Dijkwel},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2015},
  volume={124},
  pages={295-306}
}
The ability of plant tissues to retain totipotency despite being fully differentiated has been documented for decades. The transition from mature plant tissue to rejuvenated tissue first requires dedifferentiation of mature tissue, followed by rejuvenation (re-entry into the cell cycle) and somatic embryogenesis. We used a Nicotiana tabacum protoplast-based culture system to elucidate the role played by redox and phytohormone networks during the process of dedifferentiation and rejuvenation… CONTINUE READING

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