Redesigning the body plan of Drosophilaby ectopic expression of the homoeotic gene Antennapedia

@article{Schneuwly1987RedesigningTB,
  title={Redesigning the body plan of Drosophilaby ectopic expression of the homoeotic gene Antennapedia},
  author={Stephan Schneuwly and Roman Klemenz and W. J. Gehring},
  journal={Nature},
  year={1987},
  volume={325},
  pages={816-818}
}
Genetic and molecular studies on the expression of Antennapedia (Antp) have suggested that this gene specifies mainly the second thoracic segment. On the basis of our molecular analysis of dominant gain-of-function mutants we have postulated that the transformation of antennae into second legs is due to the ectopic overexpression of the Antp+ protein. This hypothesis was tested by inserting the complementary DNA encoding the normal Antp protein into a heat-shock expression vector and subsequent… Expand
Head and thoracic transformations caused by ectopic expression of Antennapedia during Drosophila development
TLDR
Comparison of the homeotic transformations in, and Western analysis of, different lines suggests that a relatively large amount of Antennapedia protein is required to cause antenna-to-leg transformations, and argues that, in general, developmental programmes in the insect are well buffered against the effects of ectopic homeotic gene expression. Expand
Control of antennal versus leg development in Drosophila
TLDR
Four lines of evidence are presented that identify extradenticle (exd) and homothorax (hth) as antennal-determining genes and that Antp promotes leg development by repressing hth and consequently nuclear Exd. Expand
Molecular basis for the inhibition of Drosophila eye development by Antennapedia
TLDR
It is demonstrated that Antp represses the activity of the eye regulatory cascade by ectopic expression, and this inhibitory mechanism involves direct protein–protein interactions between the DNA‐binding domains of EY and ANTP, resulting in mutual inhibition. Expand
Direct control of antennal identity by the spineless-aristapedia gene of Drosophila
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Antennapedia is involved in the development of thoracic legs and segmentation in the silkworm, Bombyx mori
TLDR
The results indicated that BmAntp is responsible for the Wes mutant and has an important role in determining the proper development of the thoracic segments and the understanding of the regulation of Hox genes at different levels of expression. Expand
Mouse Hox-2.2 specifies thoracic segmental identity in Drosophila embryos and larvae
TLDR
It is found that one of the mouse Antp-like genes, Hox-2.2, when expressed in developing Drosophila cells under control of a heat shock promoter, can induce homeotic transformations that are nearly identical to those caused by ectopic expression of Antp. Expand
Autoregulation of a drosophila homeotic selector gene
TLDR
It is found that both early and persistent synthesis of the protein is required for the transformation of other body segments toward head segmental identity and implies that the Dfd protein autoactivates expression from the DFD locus during normal development. Expand
Exploring the homeobox.
TLDR
In the mouse, dominant gain and recessive loss-of-function mutations result in segmental transformations of opposite direction, as in Drosophila, which means the genetic control of the body plan is much more universal than anticipated. Expand
Ectopic expression of the Drosophila homeotic gene proboscipedia under Antennapedia P1 control causes dominant thoracic defects.
TLDR
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Dual role of the Drosophila pattern gene tailless in embryonic termini.
One of the first zygotically active genes required for formation of the terminal domains of the Drosophila embryo is tailless (tll). Expression of the tll gene is activated ectopically inExpand
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TLDR
The isolation and characterization of apparent null alleles of the Antp locus lead to a homoeotic phenotype which is the reverse of the dominant Antennapedia phenotype, namely, they result in the transformation of the second leg into an antenna but do not alter the development of the normal antenna itself. Expand
The Antennapedia gene is required and expressed in parasegments 4 and 5 of the Drosophila embryo
TLDR
Both genetic requirements and gene expression indicate that Antennapedia is the main morphological determinant in parasegments 4 and 5 of the Drosophila embryo, which suggests that interactions between different selector genes take place during wild‐type development. Expand
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TLDR
Comparison of Antp protein distribution in nervous systems from wild‐type and Df P9 embryos revealed a pattern of expression which indicated that the BX‐C represses Antp in the posterior segments with the exception of the last abdominal neuromeres (A8‐9) which are regulated independently. Expand
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TLDR
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TLDR
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TLDR
The terminal phenotype of individuals carrying lethal mutations in the W36, R11, or R14 complementation groups demonstrate that these loci are important in normal anterior development and/or body segmentation and suggest functional relationships to the homoeotic mutations previously localized to the 84A-84B1,2 polytene interval. Expand
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TLDR
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TLDR
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