Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer.

@article{Mizuno2001RedFP,
  title={Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer.},
  author={H. Mizuno and Asako Sawano and Pharhad Eli and Hiroshi Hama and Atsushi Miyawaki},
  journal={Biochemistry},
  year={2001},
  volume={40 8},
  pages={2502-10}
}
The biochemical and biophysical properties of a red fluorescent protein from a Discosoma species (DsRed) were investigated. The recombinant DsRed expressed in E. coli showed a complex absorption spectrum that peaked at 277, 335, 487, 530, and 558 nm. Excitation at each of the absorption peaks produced a main emission peak at 583 nm, whereas a subsidiary emission peak at 500 nm appeared with excitation only at 277 or 487 nm. Incubation of E. coli or the protein at 37 degrees C facilitated the… CONTINUE READING