Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy.

  title={Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy.},
  author={Kirill Kolmakov and Vladimir N Belov and Jakob Bierwagen and Christian Ringemann and V. M{\"u}ller and Christian Eggeling and Stefan W Hell},
  volume={16 1},
Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine dyes excitable with 630 nm laser light and emitting at around 660 nm have been developed. The new rhodamines are very photostable and have high fluorescence quantum yields of up to 80 %, long excited state lifetimes of 3.4 ns, and comparatively low intersystem-crossing rates. They perform very… CONTINUE READING


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