Red-emitting chimeric firefly luciferase for in vivo imaging in low ATP cellular environments.

Abstract

Beetle luciferases have been adapted for live cell imaging where bioluminescence is dependent on the cellular availability of ATP, O2, and added luciferin. Previous Photinus pyralis red-emitting variants with high Km values for ATP have performed disappointingly in live cells despite having much higher relative specific activities than enzymes like Click… (More)
DOI: 10.1016/j.ab.2017.07.001

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