Red clover necrotic mosaic virus infectious transcripts synthesized in vitro.

@article{Xiong1991RedCN,
  title={Red clover necrotic mosaic virus infectious transcripts synthesized in vitro.},
  author={Zhigang Xiong and Steven A. Lommel},
  journal={Virology},
  year={1991},
  volume={182 1},
  pages={388-92}
}
The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two essentially nonhomologous ssRNAs of 3.9 kb (RNA-1) and 1.45 kb (RNA-2) which are each capped at the 5' terminus with m7GpppA. cDNA clones short of full length by several nucleotides at both termini have been generated to both RNAs. Oligonucleotide-directed mutagenesis was employed to generate a series of RNA-1 and -2 transcription vectors in which the bacteriophage T7 RNA polymerase promoter was fused to full-length… CONTINUE READING
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