Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15.

@article{Jiang2009RecyclingOA,
  title={Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15.},
  author={Linhua Jiang and Christiane Schaffitzel and Rouven Bingel-Erlenmeyer and Nenad Ban and Philipp Korber and Roman I Koning and Dani{\"e}l C de Geus and Jasper R Plaisier and Jan Pieter Abrahams},
  journal={Journal of molecular biology},
  year={2009},
  volume={386 5},
  pages={1357-67}
}
When heat shock prematurely dissociates a translating bacterial ribosome, its 50S subunit is prevented from reinitiating protein synthesis by tRNA covalently linked to the unfinished protein chain that remains threaded through the exit tunnel. Hsp15, a highly upregulated bacterial heat shock protein, reactivates such dead-end complexes. Here, we show with cryo-electron microscopy reconstructions and functional assays that Hsp15 translocates the tRNA moiety from the A site to the P site of… CONTINUE READING