Recurrent somatic mutations in mouse antibodies to p-azophenylarsonate increase affinity for hapten.

@article{Sharon1989RecurrentSM,
  title={Recurrent somatic mutations in mouse antibodies to p-azophenylarsonate increase affinity for hapten.},
  author={Jacqueline Sharon and Malcolm L. Gefter and Lawrence J Wysocki and Michael N. Margolies},
  journal={Journal of immunology},
  year={1989},
  volume={142 2},
  pages={596-601}
}
Two mouse mAb specific for the hapten p-azophenylarsonate and encoded by the same combination of germ-line V, D, and J genes differ 200-fold in affinity for hapten. We determined the amino acid sequences of the V regions of the high affinity antibody and compared them to the published sequences of the low affinity antibody which is not somatically mutated. Of 19 amino acid substitutions, two, Ile57 and Thr58 in the H chain, also occur, either alone or together, in other somatically mutated… CONTINUE READING

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