Recovery of poly(3-hydroxybutyrate) from high cell density culture of Ralstonia eutropha by direct addition of sodium dodecyl sulfate

@article{Kim2003RecoveryOP,
  title={Recovery of poly(3-hydroxybutyrate) from high cell density culture of Ralstonia eutropha by direct addition of sodium dodecyl sulfate},
  author={Mia Kim and Kyung-suk Cho and Hee Wook Ryu and Eun Gyo Lee and Yong Keun Chang},
  journal={Biotechnology Letters},
  year={2003},
  volume={25},
  pages={55-59}
}
A simple and effective method for the recovery poly(3-hydroxybutyrate) [P(3HB)] directly from high cell density culture broth with no pretreatment steps has been developed. This method consists of direct addition of sodium dodecyl sulfate (SDS) to the culture broth, shaking, heat treatment, and washing steps. When the SDS/biomass ratio was higher than 0.4, the purity of recovered P(3HB) was over 95% for various cell concentrations of 50–300 g dry cell l−1, with the highest value of 97%. The… CONTINUE READING
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