Recovery of plants from leaf protoplasts of hybrid-poplar and aspen clones

  title={Recovery of plants from leaf protoplasts of hybrid-poplar and aspen clones},
  author={Julie A. Russell and Brent H. McCown},
  journal={Plant Cell Reports},
Leaf protoplasts were isolated from shoot cultures of two hybrid poplar clones (Populus alba × P. grandidentata ‘Crandon’, NC-5339 and P. nigra ‘Betulifolia’ × P. trichocarpa, NC-5331) and the Upright European Aspen (P. tremula ‘Erecta’) and were cultured in contact with screen discs floated in liquid medium. Protoplast culture was influenced by the growth medium of the source shoot cultures, the protoplast purification procedure, the plating density, and the presence or absence of a coconut… CONTINUE READING

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Michayluk MR (1975) Planta

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