Recovery of 16S ribosomal RNA gene fragments from ancient halite

  title={Recovery of 16S ribosomal RNA gene fragments from ancient halite},
  author={Steven Anthony Fish and Thomas J. Shepherd and Terry J. McGenity and William D. Grant},
During the last decade, sensitive techniques for detecting DNA have been successfully applied to archaeological and other samples that were a few hundred to a few thousand years old. Nevertheless, there is still controversy and doubt over claims of exceptionally ancient DNA. Additional accounts stretching back nearly a century suggest that microorganisms may survive over geological time in evaporite deposits. There is, however, often doubt over the age relationship between evaporite formation… 

Characterization of ancient DNA supports long-term survival of Haloarchaea.

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Initial results have revealed surprisingly complex population histories, and indicate that modern phylogeographic studies may give misleading impressions about even the recent evolutionary past.

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The precautions and criteria necessary to ascertain to the greatest extent possible that results represent authentic ancient DNA sequences are discussed, which highlight some significant results and areas of promising future research.

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The following method, which is a modification of a protocol published by Boom et a/.

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