Recovery from paralysis in adult rats using embryonic stem cells

  title={Recovery from paralysis in adult rats using embryonic stem cells},
  author={Deepa M. Deshpande and Yun Sook Kim and Tara Martinez and Jessica Carmen and Sonny Dike and Irina Shats and Lee L. Rubin and Jennifer Drummond and Chitra Krishnan and Ahmet Hoke and Nicholas J. Maragakis and Jeremy M. Shefner and Jeffrey D. Rothstein and Douglas A. Kerr},
  journal={Annals of Neurology},
OBJECTIVE We explored the potential of embryonic stem cell-derived motor neurons to functionally replace those cells destroyed in paralyzed adult rats. [] Key MethodMETHODS We administered a phosphodiesterase type 4 inhibitor and dibutyryl cyclic adenosine monophosphate to overcome myelin-mediated repulsion and provided glial cell-derived neurotrophic factor within the sciatic nerve to attract transplanted embryonic stem cell-derived axons toward skeletal muscle targets.

Tumor prevention facilitates delayed transplant of stem cell‐derived motoneurons

Delaying transplantation is focused on to assess reinnervation after prolonged denervation of nerve injuries resulting in prolonged periods of denervation, which results in poor recovery of motor function.

Neural progenitors for sensory and motor repair

Cell therapeutic approaches can restore sensory innervation of the spinal cord following injury and neurodegenerative conditions, which can lead to paralysis and loss of sensation.

Stem Cell Transplantation in Neurological Diseases

The potential of various cell therapies in childhood neurological diseases are discussed and stem-cell-based transplantation therapies for human neurological diseases, such as Parkinson disease, stroke or neuromuscular disorders are discussed.

Use of Stem Cells in the Regeneration of Peripheral Nerve Injuries: an Overview

This research presents a novel and scalable approach called “Smart Cities, Smart Towns” that aims to provide real-time information about how to design and implement smart cities.

Directing Differentiation of Embryonic Stem Cells Into Distinct Neuronal Subtypes

Current used approaches for generating neural stem cells, as well as specific neuronal subtypes, from mouse and human embryonic stem cells are described, based upon their demonstrated role in neurodegenerative disease.

Myocardial regeneration by stem cells: seeing the unseeable.

Correction of humoral derangements from mutant superoxide dismutase 1 spinal cord

We sought to define molecular and cellular participants that mediate motor neuron injury in amyotrophic lateral sclerosis using a coculture system.

Stem cells and spinal cord regeneration.

Stem cell based strategies for spinal cord injury repair.

As the understanding and ability to direct the differentiation of stem cells grows, specific targets and strategies to incorporate them are essential to define and multiple challenges to the use of stem cell based strategies for SCI remain.



Transplantation of Motoneuron-Enriched Neural Cells Derived from Mouse Embryonic Stem Cells Improves Motor Function of Hemiplegic Mice

This study suggests that motoneurons can be induced from ES cells, and ES cells become virtually an unlimited source of cells for experimental and clinical neural cell transplantation.

Axonal growth of embryonic stem cell-derived motoneurons in vitro and in motoneuron-injured adult rats.

ES cells replicate many of the developmental and mature features of true motoneurons and are an important biological tool to understand formation of motor units in vitro and a potential therapeutic tool to reconstitute neural circuits in vivo.

Regulation of spinal motoneuron survival by GDNF during development and following injury

The survival and growth-promoting effects of GDNF on spinal motoneurons during the period of programmed cell death and following injury are reviewed.

Developing motor neurons rescued from programmed and axotomy-induced cell death by GDNF

Glial-cell-line-derived neurotrophic factor (GDNF), a potent neurotrophicfactor that enhances survival of mammalian midbrain dopaminergic neurons16,17, rescues developing avian motor neurons from natural programmed cell death in vivo and promotes the survival of enriched populations of cultured motor neurons.

New roles for old proteins in adult CNS axonal regeneration

Neuroprotection through Delivery of Glial Cell Line-Derived Neurotrophic Factor by Neural Stem Cells in a Mouse Model of Parkinson's Disease

It is found that GDNF-NSCs prevented the degeneration of dopaminergic neurons in the substantia nigra and reduced behavioral impairment in these animals, suggesting a use for NSCs engineered to release neuroprotective molecules in the treatment of neurodegenerative disorders, including Parkinson's disease.

Positive Effect of Transplantation of hNT Neurons (NTera 2/D1 Cell-Line) in a Model of Familial Amyotrophic Lateral Sclerosis

Results suggest that hNT Neuron transplantation may be a promising therapeutic strategy for ALS.