Recovery and detection of Escherichia coli O157:H7 in surface water, using ultrafiltration and real-time PCR.

@article{Mull2009RecoveryAD,
  title={Recovery and detection of Escherichia coli O157:H7 in surface water, using ultrafiltration and real-time PCR.},
  author={Bonnie J. Mull and Vincent R. Hill},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 11},
  pages={3593-7}
}
Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) outbreaks have revealed the need for improved analytical techniques for environmental samples. Ultrafiltration (UF) is increasingly recognized as an effective procedure for concentrating and recovering microbes from large volumes of water and treated wastewater. This study describes the application of hollow-fiber UF as the primary step for concentrating EHEC O157:H7 seeded into 40-liter samples of surface water, followed by an… CONTINUE READING

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