Recovery, survival and functional evaluation by transplantation of frozen-thawed mouse germ cells.

@article{Frederickx2004RecoverySA,
  title={Recovery, survival and functional evaluation by transplantation of frozen-thawed mouse germ cells.},
  author={Veerle Frederickx and A H Michiels and Ellen Goossens and Gert de Block and Andr{\'e} C. van Steirteghem and Herman J. Tournaye},
  journal={Human reproduction},
  year={2004},
  volume={19 4},
  pages={948-53}
}
BACKGROUND Establishing a successful method for testicular stem cell transplantation of frozen-thawed testicular cells would be of immense benefit to boys with childhood cancer undergoing a sterilizing treatment. In this study, we evaluated different cryopreservation protocols in a mouse model by means of testicular germ cell transplantation (TGCT), in order to establish an optimal freezing protocol. METHODS AND RESULTS In a first series of experiments, we compared an uncontrolled protocol… CONTINUE READING

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