Recovering lost magnetization: polarization enhancement in biomolecular NMR.
@article{Favier2011RecoveringLM,
title={Recovering lost magnetization: polarization enhancement in biomolecular NMR.},
author={Adrien Favier and Bernhard Brutscher},
journal={Journal of biomolecular NMR},
year={2011},
volume={49 1},
pages={
9-15
}
}- Published 2011 in Journal of biomolecular NMR
DOI:10.1007/s10858-010-9461-5
Experimental sensitivity remains a major drawback for the application of NMR spectroscopy to fragile and low concentrated biomolecular samples. Here we describe an efficient polarization enhancement mechanism in longitudinal-relaxation enhanced fast-pulsing triple-resonance experiments. By recovering undetectable (1)H polarization originating from longitudinal relaxation during the pulse sequence, the steady-state (15)N polarization becomes enhanced by up to a factor of ~5 with respect to… CONTINUE READING
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